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1.
Nat Biotechnol 2005 Jan ; 1(23):63-8.
The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4.
Seo JS ,Chong H ,Park HS ,Yoon KO ,Jung C ,Kim JJ ,Hong JH ,Kim H ,Kim JH ,Kil JI ,Park CJ ,Oh HM ,Lee JS ,Jin SJ ,Um HW ,Lee HJ ,Oh SJ ,Kim JY ,Kang HL ,Lee SY ,Lee KJ ,Kang HS ,
Macrogen Inc., World Meridian Venture Center, 60-24, Gasan-dong, Seoul 153-781, Korea. jeongsun@macrogen.com
We report the complete genome sequence of Zymomonas mobilis ZM4 (ATCC31821), an ethanologenic microorganism of interest for the production of fuel ethanol. The genome consists of 2,056,416 base pairs forming a circular chromosome with 1,998 open reading frames (ORFs) and three ribosomal RNA transcription units. The genome lacks recognizable genes for 6-phosphofructokinase, an essential enzyme in the Embden-Meyerhof-Parnas pathway, and for two enzymes in the tricarboxylic acid cycle, the 2-oxoglutarate dehydrogenase complex and malate dehydrogenase, so glucose can be metabolized only by the Entner-Doudoroff pathway. Whole genome microarrays were used for genomic comparisons with the Z. mobilis type strain ZM1 (ATCC10988) revealing that 54 ORFs predicted to encode for transport and secretory proteins, transcriptional regulators and oxidoreductase in the ZM4 strain were absent from ZM1. Most of these ORFs were also found to be actively transcribed in association with ethanol production by ZM4.
Publication Type: Research Support, Non-U.S. Gov't;
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