|Publications on this gene:|
Yeast 2006 Oct 15; 13(23):913-9.
Gene Ontology annotation status of the fission yeast genome: preliminary coverage approaches 100%.
Aslett M ,Wood V ,
Wellcome Trust Sanger Institute, Cambridge CB10 1HH, UK.
In this review, we present an overview of the Gene Ontology (GO) structure and describe how the GO is implemented for Sz. pombe and made available via Sz. pombe GeneDB (http://www.genedb.org/genedb/pombe/). We give a detailed progress report of Sz. pombe GO annotation, providing the current status of both manual and automatic annotations. Fission yeast has at least one GO annotation for 98.3% of its genes (excluding annotations to 'unknown' terms), greater than the current percentage coverage for any other organism. Approximately 65% (3225 gene products) have at least one annotation to each of the three ontologies (biological process, cellular component and molecular function). Approximately 30% (1443 gene products) have GO terms derived directly from small-scale experiments in fission yeast, supporting the validity of fission yeast as a model eukaryote and a reference organism.
Publication Type: Review;
Nat Biotechnol 2006 Jul ; 7(24):841-7.
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A ,Arai R ,Yashiroda Y ,Shirai A ,Kamata A ,Sekido S ,Kobayashi Y ,Hashimoto A ,Hamamoto M ,Hiraoka Y ,Horinouchi S ,Yoshida M ,
Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
Cloning of the entire set of an organism's protein-coding open reading frames (ORFs), or 'ORFeome', is a means of connecting the genome to downstream 'omics' applications. Here we report a proteome-scale study of the fission yeast Schizosaccharomyces pombe based on cloning of the ORFeome. Taking advantage of a recombination-based cloning system, we obtained 4,910 ORFs in a form that is readily usable in various analyses. First, we evaluated ORF prediction in the fission yeast genome project by expressing each ORF tagged at the 3' terminus. Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1.
Publication Type: Research Support, Non-U.S. Gov't;
Comment In: Nat Biotechnol. 2006 Jul;24(7):789-90